Assessment of antiphospholipid antibodies in women with recurrent miscarriage at GMMMC, Sukkur

Objective: To determine and analysis of Antiphospholipid Antibodies in women with RecurrentMiscarriage belongs to District Sukkur

Study Design:Cross-sectionals study

Place and Duration of Study: This study was conducted in the Ghulam Muhammad Mahar Medical College, Sukkur and women attending local health clinics enrolled in this study after taking their informed consent fromMay, 2012 to August2013

Materials and Methods: In this study 205 patients were taken. Patient's whole blood was collected by disposable syringes through vein puncture technique from cubital vein and maximum of 10 ml blood was taken. An aliquot of this blood [4 ml] was transferred in the EDTA containing tube. Immediately after collection plasma was separated and stored at -40 °C for the measurement of plasma Anti phospholipid and anticardiolipin antibodies level. 5 ml blood was drawn in plain tube and allowed to clot, and then serum was separated and used for blood cholesterol, HDL, LDL and Plasma lipid concentration

Results: In the current research of district Sukkur showed the highest frequency in age group of 26 to 35 years [Table 140]. In physical parameters, BMI, calories intake and maternal history of RM of patients and control subjects showed significant [p<0.05] difference. In present study the assessment of antiphospholipids antibodies [aPL] 1.95 % which were significantly different [p<0.05] than controls. Results of anticardiolipin antibodies [aCL] showed 2.43 % prevalence in patients with recurrentmiscarriage

Conclusion: The present study indicated that antiphospholipid antibodies and anticardiolipin antibodies were found an indipanded risk for recurrent miscarriage in population of Sukkur

Prevalence and associated risk factors for syphilis in women with recurrent miscarriages

A Cross Sectional population based serological studies was conducted to determine the prevalence and associated risk factors for syphilis women with recurrent miscarriages. Patient's 5ml whole blood was collected through venepuncture technique. Data were collected by all women answered a questionnaire and by investigating blood sample VDRL test and FTA-ABS test. The study was conducted in a confidential manner and numbers were used to identify the participant. Total 256 women were included in the present study. Mean age of women was 29.4 years while range was 21 to 38 years [206/256]. Out of the 256 samples, 05 [1.9%] were positive for active syphilis. Majority belonged to low socioeconomic group, uneducated and had previous congenital anomaly. Active infection with Treponema pallidum [T.P] in women belonging to low socioeconomic level were disquieting. This is probably due to illiteracy and high proportion of unsafe sexual behavior. It is also suggestive that seropositive status is often discovered in routine serological studies during pregnancy

Analysis of syphilis and associated risk factors in pregnant women belongs to remote areas of sukkur

To determine and analysis of Syphilis and Associated Risk Factors in Pregnant Women Belongs to Remote Areas of Sukkur. Cross sectional study. This study was conducted on women belongs to peripheral areas of sukkur over a period of fifteen months, which beginning in May, 2012. Patient's 5ml whole blood was collected through venepuncture technique. Data were collected by all women answered a questionnaire and by investigating Blood Sample VDRL test and FTA-ABS test. The study was conducted in a confidential manner and numbers were used to identify the participant. Total 200 Pregnant women were included in the present study. Mean age of women was 25.4 years while range was 18 to 39 years. Out of the 200 samples, 6 [3.0%] were positive for active syphilis. Majority was belonging to low socioeconomic group of population. Active syphilis infection in pregnant women belongs to remote areas with low socioeconomic level were significantly disquieting. Alarming results of this study suggestive that seropositive status is often discovered in routine serological studies during pregnancy at health centers and efficient prolonged treatment of mother were available

Study to estimate the prevalence of malaria infection in Sukkur

The aim of the study to estimate the prevalence of malaria amongst the population with fever or history of fever at sukkur. Cross sectional study. This study conducted at laboratory Ghulam Mohammad Mahar Medical College Hospital Sukkur from January 2011 to December 2012. Total 1746 clinical suspected case of malaria were included in the study. Patients presenting with fever were screened, thick and thin blood films prepared on microscopic glass slide, stained with 5% Giemsa's stain. There were 344 cases below 11 years, 590 cases between 11 to 20 years.812 cases above 20 years. They were 960 males and 786 females, with male to female ratio 1.2:1 .The prevalence of plasmodium slide positivity was 7.9% [138/1746]. Among plasmodium slide positive, 60.5% [83/138] were positive for P.vivax and 39.5% [55/138] were positive for P. falciparum. Identification of malaria cases in early acute phase of disease is significant for proper curative treatment. Anti mosquito eradication measures should be taken for breeding places of vector with personal protection measures and awareness program for malaria should be initiated

Isolation of E-coli in UTI cases with malnutrition in children of distant rural area of Sukkur, Sindh

A cross-sectional study of isolation of Escherichia coli in urine culture of children and its correlation to childhood malnutrition induce microcytic hypochromic anaemia [I.D.A]. Cross Sectional Study. This study was carried out at the Biochemistry Department, GMMMC, Sukkur from 01.12.2011 to 31.05.2013. Study was carry out among the children under 05 [five] years of age. Number of children included in this study was 150. Structured questionnaire were use to obtain the required data. Laboratory investigation of blood, urine samples was performing by standard methods and anthropometric data also collected. The results showed that Escherichia coli positive children are 64.0%, 60.5% Childs has extremely low body weight, 43.6% shows stunting and 21.7% was wasting. This study showed that there is correlation between Urinary tract infection and malnutrition. So, energy yielding nutrients containing 8 - 10 essential amino acids, fates and minerals may be supplemented to less privileged patients of remote rural area of sukkur to enhance immune status

Screening for dengue virus infection at GMMMC hospital, Sukkur

Dengue is a severe arthropod borne viral disease characterized by fever, headache, retro - orbital pain, maculopapular rash, and thrombocytopenia. Disease is transmitted from one human to another by bite of infected Aedes aegypti mosquito. Disease now a days occurs in more than 100 countries worldwide. Study designed for screening suspected or probable cases to detect specific antibodies response in early stage of disease. Cross sectional study. This study was conducted at Ghulam Muhammad Mahar Medical College Hospital, Sukkur from October, 2010 to October, 2012. Patients presenting with fever, bleeding tendencies, maculopapular rash or fever with thrombocytopenia were screened as per WHO criteria for suspected dengue infection. Blood was collected after 4 - 5 days of onset of illness. The serologic test was performed by using rapid immunochromatographic assay detection of anti dengue immunoglobulin M [IgM] antibodies. 162 cases were screened for dengue infection. 16 [9.9%] patients were positive with anti-dengue IgM. 12 patients were male and 04 were female. Platelets count was observed below 100 x 10[3] cmm[3] in patients with dengue infection. Identification of dengue virus infection in early acute phase of disease is significant for morbidity and mortality screening of suspected cases and anti-mosquito eradication measures should be taken for breading sites of vector, mass education, and awareness programs may be initiated